Review



primary human newborn foreskin fibroblasts nuff-1 cells  (GlobalStem)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    GlobalStem primary human newborn foreskin fibroblasts nuff-1 cells
    Primary Human Newborn Foreskin Fibroblasts Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human newborn foreskin fibroblasts nuff-1 cells/product/GlobalStem
    Average 90 stars, based on 1 article reviews
    primary human newborn foreskin fibroblasts nuff-1 cells - by Bioz Stars, 2026-03
    90/100 stars

    Images



    Similar Products

    primary human newborn foreskin fibroblasts nuff-1 cells  (GlobalStem)
    90
    GlobalStem primary human newborn foreskin fibroblasts nuff-1 cells
    Primary Human Newborn Foreskin Fibroblasts Nuff 1 Cells, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human newborn foreskin fibroblasts nuff-1 cells/product/GlobalStem
    Average 90 stars, based on 1 article reviews
    primary human newborn foreskin fibroblasts nuff-1 cells - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    primary newborn human foreskin fibroblasts (nuff-1  (GlobalStem)
    90
    GlobalStem primary newborn human foreskin fibroblasts (nuff-1
    <t>Fibroblasts</t> (NuFF-1) were infected (MOI= 0.5 TCID50/cell) with WT, UL33-3xF, UL33Δ1, or UL33Δ2 and analyzed at 96 hpi. (A) Whole cell lysates were probed by immunoblot with the indicated antibodies. IE1, marker of lytic infection; actin, loading control. N = 3, representative blots shown. (B) Cells were fixed, permeabilized, and UL33 localization was assessed using an antibody against the FLAG epitopes (green). mCherry (red) is shown as a marker of infection, and DAPI (blue) was used to visualize nuclei. Images were acquired at 63X magnification. N= 3, representative images shown.
    Primary Newborn Human Foreskin Fibroblasts (Nuff 1, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary newborn human foreskin fibroblasts (nuff-1/product/GlobalStem
    Average 90 stars, based on 1 article reviews
    primary newborn human foreskin fibroblasts (nuff-1 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    primary newborn human foreskin fibroblasts nuff-1  (GlobalStem)
    90
    GlobalStem primary newborn human foreskin fibroblasts nuff-1
    <t>Fibroblasts</t> (NuFF-1) were infected (MOI= 0.5 TCID50/cell) with WT, UL33-3xF, UL33Δ1, or UL33Δ2 and analyzed at 96 hpi. (A) Whole cell lysates were probed by immunoblot with the indicated antibodies. IE1, marker of lytic infection; actin, loading control. N = 3, representative blots shown. (B) Cells were fixed, permeabilized, and UL33 localization was assessed using an antibody against the FLAG epitopes (green). mCherry (red) is shown as a marker of infection, and DAPI (blue) was used to visualize nuclei. Images were acquired at 63X magnification. N= 3, representative images shown.
    Primary Newborn Human Foreskin Fibroblasts Nuff 1, supplied by GlobalStem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary newborn human foreskin fibroblasts nuff-1/product/GlobalStem
    Average 90 stars, based on 1 article reviews
    primary newborn human foreskin fibroblasts nuff-1 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fibroblasts (NuFF-1) were infected (MOI= 0.5 TCID50/cell) with WT, UL33-3xF, UL33Δ1, or UL33Δ2 and analyzed at 96 hpi. (A) Whole cell lysates were probed by immunoblot with the indicated antibodies. IE1, marker of lytic infection; actin, loading control. N = 3, representative blots shown. (B) Cells were fixed, permeabilized, and UL33 localization was assessed using an antibody against the FLAG epitopes (green). mCherry (red) is shown as a marker of infection, and DAPI (blue) was used to visualize nuclei. Images were acquired at 63X magnification. N= 3, representative images shown.

    Journal: bioRxiv

    Article Title: The Human Cytomegalovirus vGPCR UL33 is Essential for Efficient Lytic Replication in Epithelial Cells

    doi: 10.1101/2024.09.18.609710

    Figure Lengend Snippet: Fibroblasts (NuFF-1) were infected (MOI= 0.5 TCID50/cell) with WT, UL33-3xF, UL33Δ1, or UL33Δ2 and analyzed at 96 hpi. (A) Whole cell lysates were probed by immunoblot with the indicated antibodies. IE1, marker of lytic infection; actin, loading control. N = 3, representative blots shown. (B) Cells were fixed, permeabilized, and UL33 localization was assessed using an antibody against the FLAG epitopes (green). mCherry (red) is shown as a marker of infection, and DAPI (blue) was used to visualize nuclei. Images were acquired at 63X magnification. N= 3, representative images shown.

    Article Snippet: Primary newborn human foreskin fibroblasts (NuFF-1) were obtained from GlobalStem.

    Techniques: Infection, Western Blot, Marker, Control

    (A) Fibroblasts (NuFF-1) were infected (MOI= 0.5 TCID50/cell) with TB40/E- mCherry -UL33-3xF (UL33-3xF). Cells were next fixed and permeabilized over a 96 h time course, and UL33 was visualized via its FLAG epitopes (green). mCherry (red) is a marker of infection, and DAPI (blue) was used to visualize host cell nuclei. Images were acquired at 40X magnification. N = 3, representative images shown (B) Cells were infected as in (A) , with an additional, parallel culture treated with PAA post-adsorption, which was collected at 72 hpi. Cells were otherwise collected over a 96 h time course, and whole-cell lysates were immunoblotted for the FLAG epitope tag to detect pUL33-3xF. IE1 is shown as a marker of lytic infection, and actin is shown as a loading control. M, mock-infected cells, harvested at 96 hpi. N = 3, representative blots shown.

    Journal: bioRxiv

    Article Title: The Human Cytomegalovirus vGPCR UL33 is Essential for Efficient Lytic Replication in Epithelial Cells

    doi: 10.1101/2024.09.18.609710

    Figure Lengend Snippet: (A) Fibroblasts (NuFF-1) were infected (MOI= 0.5 TCID50/cell) with TB40/E- mCherry -UL33-3xF (UL33-3xF). Cells were next fixed and permeabilized over a 96 h time course, and UL33 was visualized via its FLAG epitopes (green). mCherry (red) is a marker of infection, and DAPI (blue) was used to visualize host cell nuclei. Images were acquired at 40X magnification. N = 3, representative images shown (B) Cells were infected as in (A) , with an additional, parallel culture treated with PAA post-adsorption, which was collected at 72 hpi. Cells were otherwise collected over a 96 h time course, and whole-cell lysates were immunoblotted for the FLAG epitope tag to detect pUL33-3xF. IE1 is shown as a marker of lytic infection, and actin is shown as a loading control. M, mock-infected cells, harvested at 96 hpi. N = 3, representative blots shown.

    Article Snippet: Primary newborn human foreskin fibroblasts (NuFF-1) were obtained from GlobalStem.

    Techniques: Infection, Marker, Adsorption, FLAG-tag, Control

    Multistep growth analyses were performed in epithelial cells (ARPE-19 cells) infected (MOI= 0.1 TCID50/cell) with the indicated viruses over a 30-day time course. Infected cells were collected at the indicated times, and viral titers of cell-associated virus were quantified by TCID50 using naïve NuFF-1 fibroblasts. N = 3 biological replicates; graph depicts one biological replicate, with 3 technical replicates per time point. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. * p <0.05, ** p <0.01, *** p <0.005

    Journal: bioRxiv

    Article Title: The Human Cytomegalovirus vGPCR UL33 is Essential for Efficient Lytic Replication in Epithelial Cells

    doi: 10.1101/2024.09.18.609710

    Figure Lengend Snippet: Multistep growth analyses were performed in epithelial cells (ARPE-19 cells) infected (MOI= 0.1 TCID50/cell) with the indicated viruses over a 30-day time course. Infected cells were collected at the indicated times, and viral titers of cell-associated virus were quantified by TCID50 using naïve NuFF-1 fibroblasts. N = 3 biological replicates; graph depicts one biological replicate, with 3 technical replicates per time point. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. * p <0.05, ** p <0.01, *** p <0.005

    Article Snippet: Primary newborn human foreskin fibroblasts (NuFF-1) were obtained from GlobalStem.

    Techniques: Infection, Virus, Standard Deviation

    (A) Fibroblasts (NuFF-1) or (B) epithelial cells (ARPE-19) were mock-, WT-, or UL33Δ-infected (MOI= 0.5 TCID50/cell) for the times indicated. Whole cell lysates were prepared and representative viral proteins from the IE (IE1), E (pUL44), and L (pp28) kinetic classes were assessed by immunoblot. Actin was assayed as a loading control. N=3, representative blots shown.

    Journal: bioRxiv

    Article Title: The Human Cytomegalovirus vGPCR UL33 is Essential for Efficient Lytic Replication in Epithelial Cells

    doi: 10.1101/2024.09.18.609710

    Figure Lengend Snippet: (A) Fibroblasts (NuFF-1) or (B) epithelial cells (ARPE-19) were mock-, WT-, or UL33Δ-infected (MOI= 0.5 TCID50/cell) for the times indicated. Whole cell lysates were prepared and representative viral proteins from the IE (IE1), E (pUL44), and L (pp28) kinetic classes were assessed by immunoblot. Actin was assayed as a loading control. N=3, representative blots shown.

    Article Snippet: Primary newborn human foreskin fibroblasts (NuFF-1) were obtained from GlobalStem.

    Techniques: Infection, Western Blot, Control